Extended Data Figure 6 |. Tas1 pyrophosphorylates the 3’ hydroxyl group of adenosine nucleotides.

a) ¹H (top) and ³¹P NMR (bottom) spectra of pApp. See Supplementary Table 2 for assignments. b) Negative mode electrospray mass spectra for pApp (top), ppApp (middle) and pppApp (bottom). Assignment of major peaks is shown below the spectra. c) Anion-exchange traces of Tas1_tox-catalyzed reactions with dATP or GTP as pyrophosphate donors. Arrowheads indicate 3′ pyrophosphorylation products. a-c) Data are representative of two independent experiments.