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. Author manuscript; available in PMC: 2020 May 6.
Published in final edited form as: Nature. 2019 Nov 6;575(7784):674–678. doi: 10.1038/s41586-019-1735-9

Extended Data Figure 7 |. Purified Tas1tox can use pppApp as a pyrophosphate donor to pyrophosphorylate AMP resulting in pApp formation.

Extended Data Figure 7 |

a) Anion-exchange traces of pppApp and AMP after incubation with the indicated concentrations of Tas1tox for 30 min at room temperature. A control lacking Tas1tox is shown for comparison. Chromatogram is representative of two independent experiments. b) Mechanism of quantitative conversion of ATP to pApp. Only heteroatoms that participate in the reaction mechanism of pApp formation are shown.