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. 2019 Nov 22;13:1239. doi: 10.3389/fnins.2019.01239

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Copyright © 2019 Deng, Mu, Miao, Liu, Zhou, Huang, Zhang, Wang, Yang, Qian, Wang, Guo, Zhang, Liao, Wan, Lu and Zou.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Representative photomicrographs showing CD-28K (green) and PKC (red) double immunofluorescence staining of the cerebellum in NC9W, DM9W + S, and DM9W + G groups. PKC-positive Purkinje cells double labeled with CD-28K were drastically reduced in DM9W + S (single arrow). This was accompanied by diminution of dendritic profiles in the cerebellar cortex (asterisk). In the DM9W + G group, the number of Purkinje cells showing colocalization of CD-28K and PKC was comparable to that in NC9W. Magnification: ×600. Bar = 20 μm.