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. 2019 Nov 22;13:1239. doi: 10.3389/fnins.2019.01239

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Copyright © 2019 Deng, Mu, Miao, Liu, Zhou, Huang, Zhang, Wang, Yang, Qian, Wang, Guo, Zhang, Liao, Wan, Lu and Zou.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Representative photomicrographs showing CD-28K (green) and nNOS (red) double-labeled Purkinje cells in the cerebellum in the NC9W, DM9W + S, and DM9W + G groups. Note the diminution of nNOS immunofluorescence in Purkinje cells in the DM9W + S group (single arrow) as compared with the normal control (NC9W). nNOS immunofluorescence, however, was restored to a level comparable to that of the normal in DM9W + G. Magnification: ×600. Bar = 20 μm.