Table 1. Average genomic DNA (gDNA) concentration with different number of blood spots.
Number of blood spots
used of size 6mm |
Average gDNA
concentration (ng/µl) |
Average
260/280 ratio |
Total elution
volume |
Total gDNA
Yield |
---|---|---|---|---|
6mm × 1 spot (n=10) | 3.43 | 1.93 | 30µl | 102.9 ng |
6mm × 2 spots (n=10) | 6.38 | 2.18 | 30µl | 191.4 ng |
6mm × 3 spots (n=10) | 7.23 | 2.30 | 30µl | 216.9 ng |
6mm × 4 spots (n=10) | 8.91 | 2.84 | 30µl | 267.3 ng |