Table 3. Genomic DNA (gDNA) concentration of each dried blood spot (DBS) from different number of blood spots.

Total number of blood spots used	DNA Yield (ng/µl)	260/280 ratio	Total Elution Volume	Total Yield
6mm × 1 spot	3.4	1.84	30	102
6mm × 1 spot	3.52	1.9	30	105.6
6mm × 1 spot	4.52	1.8	30	135.6
6mm × 1 spot	4.7	1.39	30	141
6mm × 1 spot	2.16	2.4	30	64.8
6mm × 1 spot	2.19	1.98	30	65.7
6mm × 1 spot	2.12	2.1	30	63.6
6mm × 1 spot	4.66	2.23	30	139.8
6mm × 1 spot	3.56	2.21	30	106.8
6mm × 1 spot	3.47	1.5	30	104.1
6mm × 2 spots	4.98	2.4	30	149.4
6mm × 2 spots	5.95	2.2	30	178.5
6mm × 2 spots	6.36	2.23	30	190.8
6mm × 2 spots	6.4	2.13	30	192
6mm × 2 spots	8.98	2.28	30	269.4
6mm × 2 spots	8.06	2.32	30	241.8
6mm × 2 spots	5.4	2.1	30	162
6mm × 2 spots	5.49	1.82	30	164.7
6mm × 2 spots	6.4	2.15	30	192
6mm × 2 spots	5.82	2.21	30	174.6
6mm × 3 spots	6.86	2.45	30	205.8
6mm × 3 spots	8.08	2.61	30	242.4
6mm × 3 spots	5.72	2.28	30	171.6
6mm × 3 spots	7.13	2.41	30	213.9
6mm × 3 spots	6.94	2.21	30	208.2
6mm × 3 spots	7.18	2.51	30	215.4
6mm × 3 spots	8.7	1.98	30	261
6mm × 3 spots	6.43	2.31	30	192.9
6mm × 3 spots	8.12	2.38	30	243.6
6mm × 3 spots	7.19	1.95	30	215.7
6mm × 4 spots	9.9	2.9	30	297
6mm × 4 spots	10.9	2.84	30	327
6mm × 4 spots	7.6	2.93	30	228
6mm × 4 spots	8.23	2.74	30	246.9
6mm × 4 spots	9.25	2.62	30	277.5
6mm × 4 spots	24	2.89	30	720
6mm × 4 spots	3.36	2.84	30	100.8
6mm × 4 spots	7.62	2.91	30	228.6
6mm × 4 spots	4.17	2.86	30	125.1
6mm × 4 spots	4.12	2.96	30	123.6