Fig. 3. Effect of luteolin and luteolin 7-O-glucoside on serum TNF-α concentration and COX-2 expression in GalN/LPS-intoxicated ICR mice.

Serum TNF-α concentration (A) and COX-2 protein expression (B) were analyzed by ELISA assay and western blot analysis, respectively. The data are representative of three independent experiments. The relative induction of COX-2 protein expression was quantified by densitometry with actin used as an internal control. The data represent the mean ± standard deviation of triplicate experiments. Values sharing the same superscript are not significantly different at P <0.05 (Duncan's multiple-range test). Lut-7-G, luteolin-7-O-glucoside; TNF, tumor necrosis factor; GalN, galactosamine; LPS, lipopolysaccharide; COX, cyclooxygenase.