Table 2.

Quality control test of six HCAs observed in grilled moose and beef steaks.

HCAs	Rt (min)	Linear Regression Equation	Linear range (μg/L)	Correlation Coefficient (R²)	LOD (μg/L)	LOQ (μg/L)	Recovery %
IQ	6.68	Y = 0.255382 + 0.00955059*X	20–100	0.9939	8.2180	27.3933	79.5
MeIQx	7.60	Y = 0.168785 + 0.00587124*X	20–100	0.9934	8.5163	28.3877	88.5
MeIQ	6.68	Y = 0.287259 + 0.0119956*X	20–100	0.9934	8.4972	28.3240	quant.
PhIP	10.86	Y = 0.440687 + 0.0133516*X	20–100	0.9873	11.8526	39.5088	98.9
Harman	13.12	Y = 0.52188 + 0.0211374*X	20–100	0.9920	9.4144	31.3812	79.9
Nor-Harman	12.74	Y = 0.427437 + 0.0170845*X	20–100	0.9912	9.8680	32.8935	63.5

HCA = Heterocyclic amine. IQ = 2-amino-3-methylimidazo[4,5-f]quinolone. MeIQx = 2-amino-3,8-dimethylimidazo[4,5-f]quinoxaline; MeIQ = 2-Amino-3,4-dimethylimidazo[4,5-f]quinolone; PhIP = 2-amino-1-methyl-6 phenylimidazo[4,5-b]pyridine; Harman = 1-Methyl-9H-pyrido[3,4-b]indole; Nor-Harman = 9H-pyrido[3,4-b]indole; TriMeIQx = 2-amino-3,4,7,8-tetramethylimidazo[4,5-f]quinoxaline. LOD = Limit of detection [17]; LOQ = Limit of quantitation [17]. N = Number of calibration standards (5, 10, 20, 50, 80, 100 μg/L). Spike level = 50 μg/L. N = 6 calibration standards.

$\text{S}\text{D}\text{o}\text{f}\text{I}\text{n}\text{t}\text{e}\text{r}\text{c}\text{e}\text{p}\text{t}=\text{S}\text{E}\text{o}\text{f}\text{I}\text{n}\text{t}\text{e}\text{r}\text{c}\text{e}\text{p}\text{t}\text{*}\sqrt{\text{N}}$

(where N = 6)

$\text{L}\text{O}\text{D}=3\frac{\text{S}\text{D}\text{o}\text{f}\text{I}\text{n}\text{t}\text{e}\text{r}\text{c}\text{e}\text{p}\text{t}}{\text{S}\text{l}\text{o}\text{p}\text{e}}$

$\text{L}\text{O}\text{Q}=10\frac{\text{S}\text{D}\text{o}\text{f}\text{I}\text{n}\text{t}\text{e}\text{r}\text{c}\text{e}\text{p}\text{t}}{\text{S}\text{l}\text{o}\text{p}\text{e}}$

$\text{P}\text{e}\text{r}\text{c}\text{e}\text{n}\text{t}\text{r}\text{e}\text{c}\text{o}\text{v}\text{e}\text{r}\text{y}\left(\text{\%}\right)=100\text{*}\frac{\text{R}\text{e}\text{c}\text{o}\text{v}\text{e}\text{r}\text{e}\text{d}\text{l}\text{e}\text{v}\text{e}\text{l}}{\text{S}\text{p}\text{i}\text{k}\text{e}\text{l}\text{e}\text{v}\text{e}\text{l}}$