Proteins with asparagine runs constitute an unusually large fraction of the Neocallimastigomycota proteome. Average amino acid run (five or more of the same amino acid consecutively in a protein) fraction per clade, ordered in decreasing GC content, in the fungal kingdom. Hydrophobic (valine, leucine, methionine and isoleucine) and bulky (phenylalanine, tyrosine and tryptophan) amino acids are noticeably absent in runs, while smaller (alanine) uncharged, polar (serine, threonine, proline, glutamine) amino acids are frequently found in runs.