Fig. 5.

(A) CAZymes in Neocallimastigomycota have more N-glycosylation motifs relative to other industrially important cellulolytic fungi. The fraction of CAZymes with a specified number of N-glycosylation motifs (N-X-S/T where X is not proline) on the x-axis in N. californiae, A. robustus and P. finnis (grouped as Neocallimastigomycota here, the other members are not shown due to their lower quality genomes), T. reesei, and A. niger. Linker regions are defined as the inter-domain regions of proteins. Neocallimastigomycota has a higher proportion of CAZymes with 2 or more N-glycosyaltion motifs than either T. reesei or A. niger. (B) Threonine is disproportionately abundant in the linker region of CAZymes in Neocallimastigomycota, suggesting O-glycosylation sites may be abundant. Amino acid fraction in all proteins with at least one CAZyme domain divided into three groups: domains, linker regions of proteins with runs (five or more of the same amino acid consecutively in a protein), and linker regions of proteins without runs. Linker regions are defined as the inter-domain regions. Serines, and especially threonines, are highly enriched in the inter-domain regions of CAZymes with runs and without runs.