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. 2019 Nov 16;28:101388. doi: 10.1016/j.redox.2019.101388

Fig. 3.

Fig. 3

Comparison of NF-κB activation in GPx2 and GPx1 kd cells. Scr, GPx1 and GPx2 kd HT-29 cells (shGPx1#1 and shGPx2#1) were supplied without (-Se) or with 50 nM sodium selenite (+Se) for 72 h. Protein expression of GPx2, GPx1, and GPx4 was detected by Western Blot (A–C). Cells were cultured with 50 nM sodium selenite for 72 h and accordingly were stimulated with or without 1 ng/mL IL-1β (D–I). TNF-α mRNA (IL-1β for 3 h, D), COX-2 (IL-1β for 6 h, E), and IκBα (IL-1β for 30 min, F) protein as well as Alox5, and Alox15 mRNA levels (IL-1β for 3 h, H–I) were measured by qPCR and normalized to Rpl13a and Oaz1 or by Western Blot, respectively. Representative Western blots are shown (G) and bands were densitometrically analyzed and normalized to β-actin or Coomassie stains. Data are given as means + SD (n = 3). *p < 0.05; **p < 0.01; ***p < 0.001 vs. scr and #p < 0.05; ##p < 0.01; ###p < 0.001 vs. shGPx2#1 analyzed by two-way ANOVA with Bonferroni's post-test.