Fig. 4.

Effect of GPx2 and GPx1 kd on lipid mediator profiles. Scr, GPx1 and GPx2 kd HT-29 cells were supplemented with 50 nM sodium selenite for 72 h. Cells were stimulated for 24 h with 1 ng/mL IL-1β, and LM profiles were analyzed by UPLC-MS/MS. Levels of selected LMs and educts are shown (A–F) together with a heatmap for the whole LM profile (G). The values and color scale of the F_i column refers to changes in the percentage of LMs vs. scr without IL-1β. The values in the other columns give the concentrations of LMs in pg per 1 × 10⁶ cells. Data are given as means + SD (n = 4). *p < 0.05; **p < 0.01; ***p < 0.001 vs. scr and ^#p < 0.05; ^##p < 0.01; ^###p < 0.001 vs. shGPx2#1 analyzed by two-way ANOVA with Bonferroni's post-test. To compare basal levels of LMs (without IL-1β treatment), one-way ANOVA with Bonferroni's post-test was applied. HHT hydroxy-heptadecatrienoic acid, HETE hydroxy-eicosatetraenoic acid, HODE hydroxy-octadecadienoic acid, HEPE hydroxy-eicosapentaenoic acid, HDHA hydroxy-docosahexaenoic acid, AA arachidonic acid, EPA eicosapentaenoic acid, DHA docosapentaenoic acid. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)