Fig. 5.

Redox parameters of GPx2 and GPx1 kd cells. Scr, GPx1 and GPx2 kd HT-29 cells were supplemented with 50 nM sodium selenite for 72 h. Cells were harvested and GPx activity was measured using different substrates (H₂O₂, HPODE, HPETE, and TBHP) with a final concentration of 50 μM (A). Numbers within the bars represent percentages in comparison to the respective scr set as 100%. For the DHR123 assay, cells were incubated with 5 μM DHR for 45 min followed by 1 h treatment with or without 5 or 10 ng/mL IL-1β (B) or 1 mM H₂O₂, 50 μM HPODE, HPETE, or TBHP (C). Values of the DHR assay were normalized to viable cells analyzed by neutral red assay. NOX1 protein levels were analyzed by Western Blot 4 h after 1 ng/mL IL-1β stimulation and normalized to Coomassie staining. Data are given as means + SD (n = 3). *p < 0.05; **p < 0.01; ***p < 0.001 vs. scr and ^##p < 0.01; ^###p < 0.001 vs. shGPx2#1 analyzed by two-way ANOVA with Bonferroni's post-test.