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. 2019 Oct 22;18:903–915. doi: 10.1016/j.omtn.2019.10.014

Figure 3.

Figure 3

miR-29a-3p Modulates TNFα-Induced VCAM-1, ICAM-1, and E-selectin Expression in pHUVECs

(A–F) The mRNA levels of VCAM-1 (A), ICAM-1 (B), and E-selectin (C) were detected, and the protein expression levels of VCAM-1 (D), ICAM-1 (E), and E-selectin (F) were analyzed by immunofluorescence staining in differentially transfected pHUVECs. The nuclei were counterstained with DAPI (blue). Each experiment was performed at least three times. pHUVECs were transfected with miR-29a-3p or/and miR-29a-3p inhibitor for 48 h and then treated with or without TNFα for 3 h (mRNA detection) or for 6 h (protein detection). A scrambled sequence was used as a negative control (NC). Error bars are defined as the SD. Scale bars, 50 μm. **p < 0.01 indicates statistically significant differences. miR-29a-3p, cells transfected with miR-29a-3p mimic; miR-29a-3p inhibitor, cells transfected with miR-29a-3p inhibitor; miR-29a-3p+inhibitor, cells transfected with miR-29a-3p mimic and its inhibitor.