Table 2.

Primers for CR1 sequence-specific amplification.

	Forward Primers			Reverse primers
	SNP	Localization	5′ → 3′ Sequence	SNP	Localization	5′ → 3′ Sequence	Fragment
Simple PCR 1	rs6656401	Intron 4	CTCTGTCTCCATCTTCTCA	Generic Primer	Intron 4	CATAGTTGTAGTTGGGGATTG	257 bp
			CTCTGTCTCCATCTTCTCG
Multi 1	rs3849266	Intron 21	CTGATGGCTTGGGGTAT	rs2274567	Exon 22	CTCAATCTGCATTGATCCAC	667 bp
			CTGATGGCTTGGGGTAC			CTCAATCTGCATTGATCCAT
Simple PCR 1	rs12034598	Intron 24	GCCTGGCATCATCAAGAAAA	rs1746659	Intron 24	GGTTAGTGTTCAGAGGCAGA	1068 bp
			GCCTGGCATCATCAAGAAAG			GGTTAGTGTTCAGAGGCAGT
Multi 2	rs3737002	Exon 26	CCATTTGCCAGTCCTAC	rs11118131	Intron 26	CAAGAAGAAGGGGTGATG	457 bp
			CCATTTGCCAGTCCTAT			CAAGAAGAAGGGGTGATA
Multi 2	rs11118167	Intron 28	GCCAATATGTGAATATTATTATCTTAT	rs17047660	Exon 29	TTCTGGAGCTGTGCATTT	746 bp
			GCCAATATGTGAATATTATTATCTTAC			TTCTGGAGCTGTGCATTC
Multi 1	rs4844610	Intron 37	CTACACAAAACAGCCTTGTA	rs12034383	Intron 37	AGATGTCCATGCCTTAAC	1080 bp
			CTACACAAAACAGCCTTGTC			AGATGTCCATGCCTTAAT
	Control primers
Simple PCR 1, 2 and muli 1		HGHf	TGCCTTCCCAACCATTCCCTTA		HGHr	CCACTCACGGATTTCTGTTGTGTTTC	431 bp
Multi 2		HLA-Ef	CGGGACTGACTAAGGGGCGG		HLA-Er	GTAGCCCTGTGGACCCTCTTAC	324 bp

In bold: variant nucleotide. SNP, single nucleotide polymorphism; bp, base pairs; HGH, human growth hormone; HLA-E, Human leukocyte E antigen; f, forward; r, reverse; Multi, multiplex PCR-SSP.