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. 2019 Nov 22;9:1283. doi: 10.3389/fonc.2019.01283

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Copyright © 2019 Li, Dai, Gong, Song, Tai and Shi.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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CDK15 does not regulate the tumor cell proliferation. (A) Expression statuses of CDK15 in three breast cancer cell lines were detected, which the cells were transfected with vector control and lenti-virus plasmid encoding CDK15. (B) CDK15 was knocked down in MCF12A cells by a set of shRNAs. (C) The effects of CDK15 over-expression on the proliferation of breast cancer cell lines were detected by CCK8 assay (NS, P > 0.05). (D) Cell-cycle analyses of breast cancer cell lines were done by flow cytometry when CDK15 was over-expressed (NS, P > 0.05). (E,F) Cell proliferation rate was detected in vector control and CDK15-knockdown MCF12A cells (E, CCK-8 assay; F, Cell cycle analyses; NS, P > 0.05).