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. 2019 Nov 22;10:2717. doi: 10.3389/fimmu.2019.02717

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Copyright © 2019 Polito, Cristantielli, Weber, Del Bufalo, Belardinilli, Arnone, Petretto, Antonucci, Giorda, Tumino, Pitisci, De Angelis, Quintarelli, Locatelli and Caruana.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Phenotype of freshly isolated and expanded γδ-T cells stimulated with aAPCs/pp65 and CD40L/pp65. Evaluation of T cells, NK cells, as well as of αβ and γδ-T cell subset distribution, in freshly isolated and expanded γδ-T cells (n = 7) (A). (B) displays the flow-cytometry analysis of the CD4⁺, CD8⁺, and CD4^neg/CD8^neg cell content within the freshly isolated and expanded γδ-T cells (n = 4). Distributions of Vδ1, Vδ2, and Vδ1^negVδ2^neg subpopulations within the CD4⁺, CD8⁺, and CD4^neg/CD8^neg in freshly isolated and expanded γδ-T cells are displayed in panels (n = 4) (C). Data summarised as average ± SEM of 4–7 donors. *p < 0.05; **p < 0.01.