Figure 4.

Functionality study of CD40L/pp65 expanded γδ-T against primary leukaemia blasts. (A) shows the long-term cytotoxic activity of 4 different γδ CD40L/pp65 T cell products against three primary acute leukaemia blasts. Panel of cytokines produced after 24 h of co-culture by multiplex analysis (B). Data from 4 donors are expressed as average ± SEM. Long-term cytotoxic activity of 4 different γδ CD40L/pp65 T cell products against primary AML blasts (C). Panel of cytokines produced after 24 h of co-culture with primary AML blasts, detected by multiplex analysis (D). (E) demonstrates the transduction efficiency of CD40L/pp65 γδ-T cell products with a retrovirus vector encoding a third generation GD2-CAR. Short-term cytotoxic activity by ⁵¹Cr-release assay against the CHLA255 neuroblastoma cell line is reported in (F). Data from 3 donors is expressed as average ± SEM.