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. 2019 Nov 28;10:353. doi: 10.1186/s13287-019-1431-6

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 6 — Cell viability of cell sheets. a, b Live–dead cell viability assay: a live (green) and dead (red) staining of cell sheet and cell suspension produced by chemical disruption. b Live cell and dead cell ratio was calculated using ImageJ. c Cell mechanosensor expression analysis of YAP proteins using western blot. YAP, pYAP, and GAPDH in whole cell lysates (10 μg protein/lane). Scale bar = 100 μm