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. 2019 Nov 28;7:327. doi: 10.1186/s40425-019-0750-6

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — APG-115 upregulates PD-L1 expression on MH-22A tumor cells. MH-22A mouse tumor cells were treated with indicated concentrations of APG-115 for 72 h in vitro. a expression levels of MDM2, p53, total STAT3 (t-STAT3), phosphorylated STAT3 (p-STAT3), PD-L1, and β-actin (loading control) were determined by Western blotting. b PD-L1 expression levels which were reflected by fluorescence intensity were determined by flow cytometry and the same results were shown as a bar chart (c)