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. 2019 Nov 29;17:159. doi: 10.1186/s12964-019-0456-x

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Stathmin is downregulated upon KPNA2 depletion. a Workflow of LC-MS/MS analysis. HLE cells were harvested 72 h after control (Ctrl.) or KPNA2 siRNA treatment (n = 3). b Volcano-Plot illustrates the resulting log₂ fold-changes (KPNA2/Ctrl. siRNA) and corresponding log₁₀ p-values of 1759 proteins being quantified by the LC-MS/MS analysis. Horizontal dotted line p = 0.01; vertical dotted lines log₂ fold-change 0.8 or − 0.8; green dot: KPNA2; red dot: stathmin. c HLE and HLF cells were siRNA-treated and harvested as described in (a). Lysates were immunoblotted using the indicated antibodies. d HLE and HLF cells were treated as described in (a) and STMN1 expression was analyzed by qRT-PCR. (HLE: n = 5, p < 0.01 (**); HLF: n = 4, p < 0.05(*))