Table 2.

Bone-marrow derived mesenchymal stem cell studies assessing post-thaw cell morphology

Study	Species	Results post-thaw	Method of assessment
Human
Kotobuki et al. [92]	Human	No effect on morphology	Microscopy (fluorescent/phase contrast)
Haack-Sorensen et al. [19]	Human	No effect on morphology	NA
Xiang et al. [93]	Human	No effect on morphology	Microscopy (light) at cell confluency post-thaw
Zhao et al. [94]	BM-MSC (human with chronic myeloid leukemia)	No effect on morphology	NA
Heng [30]	Human	The addition of Y-27632 altered the morphology of the cells (web-like appearance)	NA
Liu et al. [28]	Human	No effect on morphology	Microscopy (fluorescent)
Doan et al. [95]	Human	No effect on morphology	Microscopy (light) 7 days post
Mamidi et al. [33]	Human	No effect on morphology	NA
Moll et al. [38]	Human	Effect of cryopreservation seen on forward scatter but not side scatter when exposed to human serum	Microscopy; cell counter and analyser system (CASY-TT); Flow cytometry
Al-Saqi et al. [66]	Human	No effect on morphology	Microscopy (light) two passages post
Other species
Liu et al. [29]	Rat, mouse and calf	No effect on morphology	Microscopy (light)
Naaldijk et al. [27]	Rat	No effect on morphology	Microscopy (light)
Davies et al. [42]	Rat	No effect on morphology	Microscopy (phase contrast)
Zhu et al. [46]	Dog	Cells had several shapes such as long fusiform, polygon and astroid	Checked at days 2 and 5 after thawing NA
Edamura et al. [36]	Dog	No effect on morphology	Microscopy (light)
Mitchell et al. [32]	Horse	No effect on morphology	Microscopy (light) (24 and 72 h post)

The key results on bone-marrow derived mesenchymal stem cell morphology are presented in this table. For further details on the cryopreservation experimental details refer to either Table 1 or Additional file 2 which provide the individual freezing protocols outlined in the extracted papers alongside the concentration and passage of cells at the point of cryopreservation and the process of thawing