Table 6.

Bone-marrow derived mesenchymal stem cell studies evaluating post-thaw metabolic activity

Study	Species	Results post-thaw	Method of assessment
Human
Liu et al. [28]	Human	Reduced-DMSO freezing solution gives comparable metabolic activity to 10% DMSO	AlamarBlue assay
Chinnadurai et al. [20]	Human	No reduction in metabolic fitness	Calcium uptake; PrestoBlue reduction
Chinnadurai et al. [68]	Human	The addition of various concentrations of hPL (human platelet lysate) did not significantly enhance MSC metabolic activity	PrestoBlue reduction
Other species
Liu et al. [29]	Rat, mouse and calf	In general, non-cryopreserved cells showed higher overall metabolic activities than the cryopreserved; Reduced DMSO (5%) with 2% PEG, 3% trehalose and 2% albumin give superior results to 10% DMSO	AlamarBlue assay
Nitsch et al. [97]	Monkey	Lower metabolic activity for cryopreserved cells compared with fresh; Enhnaced levels of metabolic activity obtained for 5% and 10% DSMO levels	MTT assay (24 h, 48, 72 and 96 h post-thaw)
Lauterboeck et al. [49]	Monkey	Cells’ metabolic activity was impaired up until 48 h post-thaw; Partial recovery at 72 h and full recovery observed at 96 h	MTT assay (24 h, 48, 72 and 96 h post-thaw)

The key results on bone-marrow derived mesenchymal stem cell metabolic activity after cryopreservation are presented in this table. For further details on the cryopreservation experimental details refer to either Table 1 or Additional file 2 which provide the individual freezing protocols outlined in the extracted papers alongside the concentration and passage of cells at the point of cryopreservation and the process of thawing