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. Author manuscript; available in PMC: 2019 Nov 29.
Published in final edited form as: Proteins. 2000 Mar 1;38(4):351–360. doi: 10.1002/(sici)1097-0134(20000301)38:4<351::aid-prot1>3.0.co;2-6

TABLE I.

Fluorescence Anisotropy Decays of AFEST at Different Temperatures

T (°C) Φk(ns) a δΦk(ns) b gk a δgk b χR2c

25.0      0.17 −0.01, +0.02 0.30 0.01
25.1 −2.3, +2.6 0.70 0.01 1.50
45.0      0.05 −0.005, +0.007 0.34 0.01
   9.1 0.6 0.66 0.01 1.20
65.0      0.07   0.01 0.37 −0.02, +0.01
   5.7 −003, +0.4 0.63 −0.01, +0.02 1.10
85.0      0.03   0.01 0.52 −0.01, +0.02
   2.6 0.3 0.48 −0.02, +0.01 1.80
a

For the definition of Φkandgk see Eq. (3). For γexc = 298 nm the r0 was fixed to the value of 0.29.38

b

Standard deviations calculated by the Monte Carlo method.

c

Reduced chi-square calculated for experimental uncertainties δp = 0.2 deg and δm = 0.005.