Fig. 1.

LPS-induced special increase of MCT1, MCT4, and PFKFB3 in BV2 cell line and primary microglia. a Representative morphology of BV2 cells after treatment with PBS, LPS, or IL-4. Scale bar = 200 μm. b Quantification of the mRNA levels of classical microglial markers and glycolysis-related regulatory factors after treatment with LPS for 24 h (n = 6–8 per group and errors represent S.E.M; t test). c Quantification of the mRNA levels of alternative M2 markers and glycolysis-related regulatory factors after treatment of IL-4 for 24 h (n = 5 per group; t test). d Representative immunoblots and relative quantification of iNOS, MCT1, MCT4, and PFKFB3 after treatment with PBS, LPS, or IL-4 (n = 6 per group; one-way ANOVA). e, f Quantification of the mRNA levels of these genes in primary cultured microglia in each group (n = 6 per group one-way ANOVA). *p < 0.05, **p < 0.01 versus PBS-treated group