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. 2019 Nov 29;20:908. doi: 10.1186/s12864-019-6277-x

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — The growth arrest recovery translatome of O. dioica. Animals were cultured under dense conditions before being released by dilution in the presence of food. a Median translational efficiency (RPF/mRNA = ribosome protected fragment density/mRNA density) of mRNAs from 2 replicates for growth arrested and released animals with transcripts identified as having significantly up- or down-regulated translation highlighted. b Degree of change in translational efficiency upon release from stasis (y-axis) against log fold change in mRNA abundance (x-axis) with known mTOR-independent (histone mRNAs) and mTOR target (ribosomal protein mRNAs) gene categories highlighted. The majority of mTOR targets are not up-regulated upon release from stasis. c-g EdU incorporation (DNA replication: green) was restored in the germline (top in each image, as indicated by arrows) 12 h after animals were released from the crowded conditions of growth arrest independent of food supply confirming that a change in density rather than increased food availability is the primary trigger for exiting a growth-arrested state in O. dioica (DNA was counterstained with blue To-Pro-3 iodide). c normal day 3; d growth arrest; e release without food; f release with food; g number of animals showing extensive DNA synthesis under all four conditions (C = normal day 3 control; S = stasis; R = release without food; RF = release with food)