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ox-LDL induction in the PAR2 expression in VSMCs. (A, B) Cells were treated with ox-LDL (100 μg/ml) for 24 h. qRT-PCR and Western blotting were performed to estimate the expression of PAR2. ** p<0.01 versus control. (C, D) Cells were transfected with PAR2-pcDNA3.1 or NC for 48 h. Transfection efficacy was detected by qRT-PCR and Western blotting. NC, pcDNA3.1 vector as the negative control. ** p<0.01, *** p<0.001 versus control or NC.
