Fig. 1.

No replication defect in vitro of IIV6 lacking its RNAi suppressor protein 340R. (A) Strategy to generate an RNAi suppressor-defective IIV6 mutant (IIV6 Δ340R). The recombination template plasmid contained the GFP transgene fused to 14 N-terminal amino acids of 340R, flanked by homology arms at both the 5′ and 3′ ends. (B) Replication kinetics of IIV6 WT and Δ340R in Drosophila S2 cells. Titers (Left) are presented as means ± SD of 3 biological replicates. qPCR data (Right) were normalized to housekeeping gene rp49 and presented relative to the 12 hpi time point (log-transformed data). qPCR data are means of 2 technical replicates.