Fig. 1.

aPKCi overexpression triggers basal extrusion of luminal cells in vivo. (A) Scheme of mouse mammary organoid transplantation experiments. (B) Confocal images of regenerated mammary glands from infected mouse organoids. Myoepithelial and luminal cells were stained with anti–cytokeratin-5 (cyan) and anti–cytokeratin-8 (magenta) antibodies, respectively. (C) Quantification of GFP⁺ cells breaching the myoepithelial cell layer. A Fisher test was performed. (D) Confocal images of regenerated mammary glands from infected mouse organoids. The basement membrane (BM) and epithelial cells were stained with anti–laminin-5 (cyan) and anti–E-cadherin (magenta) antibodies, respectively. The white arrowheads show GFP-aPKCi cells escaping from the duct. (E) Quantification of GFP⁺ cells breaching the BM. A Fisher test was performed. Nuclei were stained with DAPI (blue in B and D). (Scale bars, 10 µm.)