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. 2019 Nov 12;116(48):24049–24055. doi: 10.1073/pnas.1908364116

Fig. 3.

Fig. 3.

(A) Weblogo analysis (45) of 400 aligned leader sequences demonstrates the conserved motif. (B) Sequences of the McjA peptide and the engineered McjAYxxP peptides. The 3 mutated residues in McjAYxxP are indicated in red. (C and D) Competitive binding assays for the (C) truncated McjA leader peptide and (D) truncated McjAYxxP leader peptide indicate that the orthogonal McjA leader peptide cannot bind, and insertion of the conserved binding motif is sufficient to binding to TbiB1. (E and F) MALDI-TOF-MS analysis of protease action observing (E) McjA and McjAYxxP leader peptide and (F) McjA and McjAYxxP core peptide. (G and H) MALDI-TOF-MS analysis of TbiB1- and TbiB2-dependent processing of (G) McjAYxxP leader peptide and (H) McjAYxxP core peptide.