Table 2.
sRNA-seq differential expression results for the top 15 significantly deregulated (P < 0.05) mature tRFs in aggressive CLL vs. normal controls
| tRF MINTmap ID | Fragment sequence, 5′-3′ | Length, nt | tRF type | Linear fold change | P value | ||
| Aggressive vs. normal | Indolent vs. normal | Aggressive vs. normal | Indolent vs. normal | ||||
| tRF-34-5YXENDBP1IUU1J | GATTGTGAATCTGACAACAGAGGCTTACGACCCC | 34 | i-tRF | 2,053.45 | 1,499.29 | 0.00030 | 0.00114 |
| tRF-20-V25Z2IUI | TAGGAGATTTCAACTTAACT | 20 | i-tRF | 1,927.74 | 1,409.13 | 0.00030 | 0.00114 |
| tRF-18-5YXENDR | GATTGTGAATCTGACAAC | 18 | i-tRF | 1,786.08 | 2,329.87 | 0.00030 | 0.00030 |
| tRF-33-5YXENDBP1IUUDJ | GATTGTGAATCTGACAACAGAGGCTTACGACCC | 33 | i-tRF | 1,339.26 | 954.66 | 0.00030 | 0.00114 |
| tRF-27-08F4BDNZ8O4 | ACCGGAGATGAAAACCTTTTTCCAAGG | 27 | i-tRF | 1,129.54 | 822.20 | 0.00909 | 0.00209 |
| tRF-19-5YXEND0U | GATTGTGAATCTGACAACA | 19 | i-tRF | 1,110.67 | 1,401.93 | 0.00030 | 0.00030 |
| tRF-16-5YXENDB | GATTGTGAATCTGACA | 16 | i-tRF | 893.64 | 757.54 | 0.00042 | 0.00042 |
| tRF-25-HMI8W47W1R | ATATCATTGGTCGTGGTTGTAGTCC | 25 | i-tRF | 837.55 | 788.87 | 0.00035 | 0.00035 |
| tRF-20-2IUIX1Q7 | CAACTTAACTTGACCGCTCT | 20 | i-tRF | 837.47 | 618.80 | 0.00039 | 0.00039 |
| tRF-25-M0IBB7Z92K | CGGAGATGAAAACCTTTTTCCAAGG | 25 | i-tRF | 835.19 | 661.14 | 0.00030 | 0.00379 |
| tRF-17-FN7BWU2 | AGATTGTGAATCTGACA | 17 | i-tRF | 808.16 | 870.72 | 0.00030 | 0.00030 |
| tRF-16-B2Y0FDD | AAAGATTAAGAGAACC | 16 | i-tRF | 764.07 | 1,142.20 | 0.00030 | 0.00030 |
| tRF-26-325UB1ZZK00 | CCGGAGATGAAAACCTTTTTCCAAGG | 26 | i-tRF | 687.57 | 556.40 | 0.00030 | 0.00379 |
| tRF-19-H6Z3NPIJ | ATCCATTGGTCTTAGGCCC | 19 | i-tRF | 676.43 | 876.26 | 0.00030 | 0.00379 |
| tRF-21-DNB2Y0FDD | AAGTTAAAGATTAAGAGAACC | 21 | i-tRF | 622.10 | 579.68 | 0.00030 | 0.00030 |
| tRF-26-KVNMEH623K0 | CCCGCCTGTCACGCGGGAGACCGGGG | 26 | i-tRF | −52.97 | −48.61 | 0.00024 | 0.00018 |
| tRF-16-RK9P4PE | GGGGGTGTAGCTCAGT | 16 | 5′-tRF | −55.60 | −133.42 | 0.00055 | 0.00044 |
| tRF-18-OUR83004 | GACGAGGTGGCCGAGTGG | 18 | 5′-tRF | −59.07 | −20.03 | 0.00005 | 0.00137 |
| tRF-16-YU4RRW0 | TTCGACTCCCGGTGTG | 16 | i-tRF | −69.74 | −20.86 | 0.00039 | 0.00005 |
| tRF-19-RKVP4PJZ | GGGGGTATAGCTCAGTGGT | 19 | 5′-tRF | −74.65 | −44.26 | 0.00035 | 0.00035 |
| tRF-20-RK9P4P9L | GGGGGTGTAGCTCAGTGGTA | 20 | 5′-tRF | −76.64 | −258.08 | 0.00042 | 0.00024 |
| tRF-17-YU4RRQ4 | TTCGACTCCCGGTATGG | 17 | i-tRF | −84.37 | −12.90 | 0.00034 | 0.00213 |
| tRF-23-RK9P4P9LDS | GGGGGTGTAGCTCAGTGGTAGAG | 23 | 5′-tRF | −84.39 | −225.24 | 0.00035 | 0.00024 |
| tRF-22-RKVP4P9LL | GGGGGTATAGCTCAGTGGTAGA | 22 | 5′-tRF | −89.79 | −273.41 | 0.00042 | 0.00030 |
| tRF-22-PZK0JNOR4 | GCCTTCCAAGCAGTTGACCCGG | 22 | i-tRF | −141.65 | −33.28 | 0.00018 | 0.00024 |
| tRF-17-31QJ3KQ | CCGCCGCGGCCCGGGTT | 17 | i-tRF | −152.75 | −40.41 | 0.00018 | 0.00024 |
| tRF-38-RPFQRDWXHR13M80E | GGTAGAGCATGGGACTCTTAATCCCAGGGTCGTGGGTT | 38 | i-tRF | −161.18 | −201.05 | 0.00012 | 0.00018 |
| tRF-21-RKVP4P9L0 | GGGGGTATAGCTCAGTGGTAG | 21 | 5′-tRF | −188.88 | −126.57 | 0.00030 | 0.00030 |
| tRF-16-RKVP4P0 | GGGGGTATAGCTCAGG | 16 | 5′-tRF | −242.56 | −222.28 | 0.00042 | 0.00039 |
| tRF-18-7SERM404 | GTTCGACTCCCGGTGTGG | 18 | i-tRF | −314.03 | −54.18 | 0.00018 | 0.00024 |
Corresponding indolent CLL vs. normal control fold changes are reported. i-TRF, wholly internal tRF.