Fig. 6.
Binding of the Dsl1 lasso to αCTD is essential in sensitized yeast. (A) Alleles of Dsl1, provided on a leucine prototrophy plasmid, were assayed for viability upon loss of a wild-type Dsl1 plasmid (Right). A plating control was included to quantify leucine plasmid maintenance (Left). (B) Alleles of α-COP, provided on a histidine prototrophy plasmid, were assayed for viability in combination with the dsl1ΔE allele (Right). A plating control was included to quantify histidine plasmid maintenance (Left).