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. 2019 Nov 8;116(48):24184–24195. doi: 10.1073/pnas.1913307116

Fig. 3.

Fig. 3.

Characterization of the prometastatic effects of the miR-23b cluster. (A–D) PanNET experimental liver metastasis assays via tail vein injections using miR-23b cluster overexpressing βTC3 cells with distinctive regimens of DOX-induced expression. (A) DOX administration schedules. (B) Survival of mice variably induced to express the miR-23b cluster. n = 5. Log-rank test. ***P < 0.001. (C) Bioluminescence imaging of representative mice at day 30 for the 4 induction schedules (radiance; p/sec/cm2/sr). (D) Time course of the bioluminescence signal in the liver during the expansion phase. (E and F) PanNET experimental liver metastasis assays via intrahepatic injection using control βTC3 cells or miR-23b cluster overexpressing cells. Quantification of apoptosis (E; Tag+/Cleaved Caspase 3) and proliferation (F; Tag+/EdU) of βTC3 control and miR-23b cluster overexpressing cells, 24 h after intrahepatic injection. n = 3 to 8. Student’s t test. *P < 0.05, ***P < 0.001.