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. Author manuscript; available in PMC: 2020 Jul 16.
Published in final edited form as: Immunity. 2019 Jun 24;51(1):155–168.e5. doi: 10.1016/j.immuni.2019.05.006

Figure 4. Substantial differences in SJL genetic content are present in B6.SJL-CD45.1 mice from different vendors.

Figure 4.

(A, B) CD4+ T cells were isolated from B6-CD45.2, B6.SJL-CD45.1, and SJL mice from Jax (blue highlight), B6N-CD45.2 and B6.SJL-CD45.1 mice from Charles River (purple highlight), or B6.SJL-CD45.1 mice from Taconic (green highlight) and cells were polarized in Th1 conditions. RNA-seq datasets were processed with default (mismatches; blue, purple, or green lettering) or stringent (0 mismatches; red lettering) parameters. Shown are heatmaps representing the z scores for the normalized counts from the DESeq2 analyses of the indicated samples from (A) Jax or (B) CR, Tac and Jax mice. See methods for heatmap gene selection. Datasets from at least three independent biological replicates are shown. (C) Schematic of chromosomes 1, 7, and 19. Blue (Jax), purple (CR), and green (Tac) boxes indicate the regions defined to contain SJL genetic content in B6.SJL-CD45.1 mice from each vendor. White dashed line represents a region detected to have B6 content in H3K27Ac analysis for genetic variation. See also Figs. S3–5.