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. Author manuscript; available in PMC: 2020 Jul 16.
Published in final edited form as: Immunity. 2019 Jun 24;51(1):155–168.e5. doi: 10.1016/j.immuni.2019.05.006

Figure 6. Genetic variation is found in H3K27Ac elements in CD4+ Th1 cells.

Figure 6.

(A) Heatmap of H3K27Ac ChIP-seq datasets comparing default and stringent alignment parameters for CD4+ Th1 cells isolated from B6-CD45.2 and B6.SJL-CD45.1 mice from Jax or CR. See results and methods for heatmap peak selection. (B) UCSC genome browser display of H3K27Ac ChIP-seq tracks for CD4+ Th1 cells isolated from B6-CD45.2 or B6.SJL-CD45.1 mice from Jax. Viral LTR annotation is shown below the tracks. Primer locations for PCR in (C) are shown with blue arrows. (C) PCR analysis examining DNA from B6-CD45.2 or B6.SJL-CD45.1 mice from Jax. (A-C) Data are representative of at least 2 independent biological replicates. See also Fig. S5.