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. 2019 Nov 25;12:10115–10127. doi: 10.2147/OTT.S226830

Figure 4.

Figure 4

Silencing of p21 neutralized the PPI-inhibited cell proliferation but had no effect on feedback regulation of HOTAIR and c-Jun expressions. (A, B) PC9 and H1650 cells were transfected with the control or p21 siRNAs for 24 h before exposure of the cells to PPI for an additional 24 h, followed by detecting HOTAIR levels and c-Jun protein expression via qRT-PCR and western blot, respectively. (C) PC9 and H1650 cells were transfected with the control or p21 siRNAs for 24 h before exposure of the cells to PPI (1.6 μM) for an additional 24 h, followed by detecting cell proliferation via the MTT assay described in the Materials and Methods section. The figures are representative cropped gels/blots that have been run under the same experimental conditions. Values in bar graphs were given as the mean±SD from three independent experiments. *Indicates significant difference as compared to the untreated control group (P<0.05). **Indicates significant difference from PPI treated alone (P<0.05).