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. 2019 Nov 7;42(11):794–803. doi: 10.14348/molcells.2019.0121

Fig. 3. Hoxa13 mediates the function of lncRNA-HOTTIP in UV-stimulated GC-1 cells.

Fig. 3

(A–D) Overexpression of Hoxa13 blocks the inhibitory effects of si-HOTTIP on G2/M phase arrest (A and B) and early apoptosis (C and D). GC-1 cells were transfected with either si-NC or si-HOTTIP and pcDNA3.1(+) or pcDNA3.1(+)-Hoxa13, and were treated with 0 and 5 J/m2 UV 24-h post transfection. 24-h post UV irradiation, cell cycle progression (A and B) and apoptosis (C and D) of GC-1 cells were assessed using FACS. Representative photographs of cell cycle progression and apoptosis were shown (B) and (D), respectively. (E) Hoxa13-mediates the promotion of expression of p53, p21 and γ-H2AX proteins, which are reduced in si-HOTTIP post UV exposure in GC-1 cells. GC-1 cells were transfected with si-NC or si-HOTTIP, and were irradiated with and 5 J/m2 UV 24-h post transfection. Isolated proteins was analyzed using western blotting. (A and C) Data shown are the mean ± SEM of three independent experiments each performed in triplicate. *P < 0.05.