Table 1:
Description | Genomic DNA | BAC clones |
---|---|---|
Sequencing and assembly data | ||
Sequencing data | 26 Illumina synthetic long-read libraries | Single-end Roche 454 of BAC library clones |
Total sequence (Gb) | 19 | 6.6 |
Genome coverage | 1.9× | 0.66× |
Read length minimum/maximum/mean (bp) | 1,500/22,904/4,930 | 8/2,611/368.5 |
Assembler software | Celera Assembler (Overlap Graph) | PHRAP/CONSED |
Total reads used in assembly | 3,857,849 | 17,894,306 |
Total assembly size | 4.26 Gb | 49.6 Mb |
Number of unitigs/contigs + singletons | 450,609 | 463 |
Contigs length minimum/maximum/mean (bp) | 1,500/468,011/9,452 | 11,723/235,533/107,129 |
NG50 (bp) | 41,394 | 109,618 |
N50 (bp) | 13,157 | N/A |
Gene prediction features | ||
No. genes | 373,869 | 3,550 |
No. transcripts | 374,774 | |
No. exons | 1,035,764 | 13,132 |
Mean GC content (%) | 43.20 | 44.99 |
Mean No. exons per gene | 2.8 | 3.7 |
Mean exon size (bp) | 291 | 271.8 |
Median exon size (bp) | 171 | 154 |
Mean intron size (bp) | 352.6 | 539.2 |
Median intron size (bp) | 132 | 139 |
Mean gene size (bp) with UTR | 1,437.80 | 2,429.20 |
Median gene size (bp) with UTR | 806 | 1,260.50 |
Mean gene size (bp) without UTR | 1,318.80 | 2,351.30 |
Median gene size (bp) without UTR | 771 | 1,199.50 |
Mean gene density (kb per gene) | 11.4 | 14 |
GC: guanine-cytosine; UTR: untranslated region.