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. Author manuscript; available in PMC: 2020 Jul 1.
Published in final edited form as: Metab Eng. 2019 May 7;54:286–291. doi: 10.1016/j.ymben.2019.04.011

Figure 3.

Figure 3.

In vitro protease protection assay. a) Schematic of carboxypeptidase A (CPA) protection assay for purified CPHhis6-T1-PSII shells incorporating CPH-GFPhis6 (top) or WTH-GFPhis6 (bottom). For clarity, only one CPHhis6 (cyan) and CPH-GFPhis6 (yellow) or WTH-GFPhis6 (pink) is shown per shell. b) Western blot of a CPA time course of purified CPHhis6-T1-PSII shells incorporating CPH-GFPhis6 (top) or WTH-GFPhis6 (bottom) using anti-his antibodies. Representative in-blot intrinsic GFP fluorescence is shown below each western blot. See Figure S3 for uncropped fluorescence images.