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. Author manuscript; available in PMC: 2020 Apr 19.
Published in final edited form as: ACS Synth Biol. 2019 Mar 27;8(4):668–674. doi: 10.1021/acssynbio.9b00011

Figure 2. Purification and characterization of SUMO-BMC-H2.

Figure 2.

A: Purification and cleavage of SUMO-BMC-H2 (37 kDa) into BMC-H2 (22 kDa) and His-SUMO (15 kDa). Lane 1: Ni-NTA elution; Lane 2: completed cleavage reaction and Lane 3: Ni-NTA flow-through containing pure BMC-H2. B: Size-exclusion chromatography elution profile of BMC-H2 corresponding to protein in lane 3 from (A). Protein molecular weight standards (red) were used to estimate the molecular weight of each species in the purified BMC-H2 sample; a:>670 kDa, b:356 kDa, c:188 kDa, d:60 kDa).