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. Author manuscript; available in PMC: 2020 Apr 19.
Published in final edited form as: ACS Synth Biol. 2019 Mar 27;8(4):668–674. doi: 10.1021/acssynbio.9b00011

Figure 4. Characterization of in vivo assembled BMC-H2 shells in the presence of BMC-PHis.

Figure 4.

A: SDS-PAGE analysis of concentrated HisTrap elution fractions. A common contaminant from the HisTrap is indicated by an asterisk. B: Size distribution of concentrated HisTrap elution fractions from (A) using Dynamic Light Scattering. C: TEM micrograph of the sample in (A). Diameter = 26 ± 2 nm (n=407). Scale bar = 100 nm. D: SDS-PAGE analysis showing the removal of excess pentamer with a size exclusion step (left two lanes) and samples purified from strains expressing BMC-Phis at two different induction levels.