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. Author manuscript; available in PMC: 2020 Apr 1.
Published in final edited form as: Leukemia. 2019 Mar 26;33(10):2429–2441. doi: 10.1038/s41375-019-0454-4

Figure 5. GSK-3β inhibition alters extrinsic apoptosis genes.

Figure 5.

A) Volcano plot of KEGG apoptosis genes derived from RNA-Seq data on Ramos cells treated for 3 hours with 3 μM CHIR99021 in biological triplicates. Key apoptotic genes are labeled, with genes in blue being down-regulated, genes in red being up-regulated, and genes in green having no significant changes. B) qRT-PCR expression analysis for known Myc-dependent intrinsic/mitochondrial apoptotic factors was performed on Ramos cells treated for 2 hours with DMSO (blue bars) or 3 μM CHIR99021 (red bars) followed by a 6 hour time course of .25 μM doxorubicin. C) qRT-PCR expression analysis for extrinsic apoptosis factors was performed on Ramos cells treated as in B). Notable changes are indicated with yellow arrows.