Skip to main content
. 2019 Oct 7;25(11):717–728. doi: 10.1093/molehr/gaz049

Figure 4.

Figure 4

Effect of F0 diet (chow versus HF/HS) on mitochondria and lipid in offspring blastocysts. (A and E) Representative images of blastocysts derived from chow and HF/HS groups. Quantification of mitochondrial mass (MTDR), mitochondrial membrane potential (MTRC/MTDR) and lipid droplets (BODIPY 409/503) in blastocysts derived from F0 (B), F1 (C) and F2 (D) oocytes (n = 29–30 blastocysts derived from five mice/diet/generation). Quantification of Oma1 protein expression in blastocysts derived from F0 (F), F1 (G) and F2 (H) oocytes (n = 25–30 blastocysts derived from five mice/diet/generation). Scale bar = 10 μm. Data are represented as mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.