Skip to main content
. 2019 Oct 7;25(11):717–728. doi: 10.1093/molehr/gaz049

Figure 8.

Figure 8

Effect of F0 diet (chow versus HF/HS) on blastocyst mitochondrial parameters and fat content with 1 μM mito-esculetin supplementation during IVF. (A and D) Representative images of blastocysts derived from chow and HF/HS groups. (B) Quantification of mitochondrial mass (MitoTracker Deep Red), mitochondrial membrane potential (MitoTracker CMXRos) and lipid droplets (BODIPY 409/503) in blastocysts derived from chow (n = 27 blastocysts from five mice) and HF/HS (n = 30 blastocysts from five mice) groups. (C) Quantification of ATP and citrate in blastocysts derived from F0 oocytes (n = 30 blastocysts derived from five mice/treatment). (E) Total cell number, apoptotic cell number and apoptosis rate in blastocysts derived F0 oocytes (n = 28–31 blastocysts derived from five mice/treatment). Scale bar = 10 μm. Data are represented as mean ± SEM. *P < 0.05, **P < 0.01, ****P < 0.0001.