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. 2019 Nov 29;9:17979. doi: 10.1038/s41598-019-54262-3

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Morphology (H&E staining) and areas of endolymphatic space in each cochlear turn at P 30. (A) Sectional images of the Slc26a4^+/− mice (a–c), the untreated side of Slc26a4^−/− mice (d-f) and the treated side of Slc26a4^−/− mice (g–i) at P 30. The areas of endolymphatic space of the treated side of Slc26a4^−/− are smaller than that of the untreated side of Slc26a4^−/−. Scale bars represent 100 μm. (B) The graph shows the areas of endolymphatic space in each cochlear turn at P 30. Each area of three turns in the treated cochleae is significantly reduced when compared with that in the untreated side. n = 5. ^*P < 0.05. ^**P < 0.01 (Mann-Whitney U).