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. 2019 Nov 29;9:17982. doi: 10.1038/s41598-019-54181-3

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Probability distribution for ORF lengths in narnavirus-like sequences. Shading shows distribution of ORF lengths coloured by reading frame after codon permutation test on RdRp coding sequences of 42 representative narnaviruses as in Fig. 3. In brief, codons are randomly re-ordered and then ORF lengths in the 5 alternate frames are calculated (permutation methods as in³³). Points give lengths of actual ORFs in reference sequences, coloured according to reading frame, with the reference RdRp as +0 frame (red, below). Note that some annotated RdRp coding regions in the database may be fragments of the complete coding sequence.