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. 2019 Nov 29;9:17999. doi: 10.1038/s41598-019-54533-z

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The mRNA relative expression levels of osteogenic genes, included Runx2 (a), Collagen I (b), Osteonectin (c), and Osteopontin (d) were determined by qRT-PCR for all samples. a: statistically significant difference compared with uncoated group at p < 0.05; (b) statistically significant difference compared with S1 group at p < 0.05; (c) statistically significant difference compared with S2 group at P < 0.05. (d) Statistically significant difference compared with S3 group at p < 0.05; (e) statistically significant difference compared with TCP group at p < 0.05.