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. 2019 Nov 29;9:17963. doi: 10.1038/s41598-019-54292-x

Figure 4.

Figure 4

Cell lineage immunophenotyping in ALI-HBEC by immunofluorescence. (A,B) Immunofluorescence for MUC5AC (yellow staining and pink quantification mask), ß-tubulin IV (red staining and quantification mask) and p63 (green staining and quantification mask) in ALI-HBEC from one control non-smoker (A) and one severe COPD patient (B). Nucleus are stained in blue by DAPI. (C) Quantification of MUC5AC staining in ALI-HBEC expressed in percentage of positive area (n = 10). (D) Quantification of ß-tubulin IV staining in ALI-HBEC expressed in percentage of positive area (n = 10). (E) Quantification of p63 staining in ALI-HBEC expressed in percentage of positive area (n = 10). Scale bar, 50 µm. White dots represent non-smoker controls and black dots current smoker controls and black squares represent severe and very severe COPD.