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. 2019 Nov 29;9:17963. doi: 10.1038/s41598-019-54292-x

Figure 6.

Figure 6

Short-term effect of TGF-β1 on epithelial cell lineages in control ALI-HBEC. (A) IHC for MUC5AC (goblet cells), ß-tubulin IV (ciliated cells) and p63 (basal cells) in ALI-HBEC without or with 72 h treatment of TGF-ß1 (10 µg/ml). (B) Quantification of MUC5AC staining in ALI-HBEC treated by TGF-ß1 expressed in percentage of positive cells (n = 4). (C) Quantification of ß-tubulin IV staining in ALI-HBEC treated by TGF-ß1 expressed in percentage of positive cells (n = 4). (D) Quantification of p63 staining in ALI-HBEC treated by TGF-ß1 expressed in percentage of positive cells (n = 5). Scale bar, 50 µm. Friedman test and Dunn’s multiple comparison test.