Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Nov 29;9:17963. doi: 10.1038/s41598-019-54292-x

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Long-term effect of TGF-β1 and anti-TGF-β1 antibody on epithelial cell lineages. (A) IHC for MUC5AC (goblet cells), ß-tubulin IV (ciliated cells) and p63 (basal cells) in ALI-HBEC using TGF-β1 (10 ng/ml), anti-TGF-β1 antibody (10 µg/ml) and control mouse IgG (10 µg/ml) during the 2 weeks of ALI differentiation (pictures are from a control ex-smoker). (B) Quantification of MUC5AC staining expressed in percentage of positive cells (n = 6), including 4 controls and 2 COPD donors as white and black dots, respectively). (C) Quantification of ß-tubulin IV staining expressed in percentage of positive cells (n = 6, including 4 controls and 2 COPD donors as white and black dots, respectively). (D) Quantification of p63 staining expressed in percentage of positive cells (n = 6, including 4 controls and 2 COPD donors as white and black dots, respectively). (E) Immunoblot for FOXJ1 (50 kDa), ß-tubulin IV (50 kDa), phospho-Smad2/3 (68 kDa) and GAPDH (37 kDa) expression in cellular lysates of ALI-HBEC from controls and COPD patients (n = 6). (F) IHC for involucrin (squamous cells) in ALI-HBEC using TGF-β1 (10 ng/ml), anti-TGF-β1 antibody (10 µg/ml) and control mouse IgG (10 µg/ml) during the 2 weeks of ALI differentiation. (G) Quantification of involucrin staining expressed in percentage of positive cells (n = 6, including 4 controls and 2 COPD donors as white and black dots, respectively, pictures are from a control ex-smoker. Scale bar, 50 µm. Friedman test and Dunn’s multiple comparison test.